The entire optical path of the BX53 is designed for optimal fluorescence imaging, using UIS2 components that set new standards in precision and clarity. High-quality mirror cube coatings provide excellent transmission and steep cut-offs, while almost all stray light is eliminated from interior surfaces for the best sensitivity and color separation. Other advanced features further facilitate fluorescence microscopy, including a new fly-eye lens system, which delivers homogenous illumination, and automatic magnification and filter cube read-out, which simplifies the repetition of settings. Additionally, the BX53 semi-motorized fluorescence microscope makes highly efficient imaging possible. Automation enables the imaging of large areas in high magnification and also facilitates multicolor fluorescence.